Behavior of the Serum proteins in starch-agar-gel electrophoresis

Authors

  • A. Amin
  • H. MANHOURI
  • K. D. SHAMLOO
  • M. KOROUR
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Starch-gel electrophoresis of rat-serum proteins.

In 1955, Smithies (1) described a procedure for the separation of human serum proteins by horizontal zone electrophoresis in starch gels. The separation of zones was improved by a later modification to a vertical electrophoresis in which the sample was inserted in the gel, free from supporting medium (2). With these methods, it has been demonstrated that many more protein fractions can be separ...

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An improved procedure for starch-gel electrophoresis: further variations in the serum proteins of normal individuals.

Experience with the method of zone electrophoresis in starch gels (Smithies, 1955) suggests that the resolution of protein components is lessened by the use of supporting substances (e.g. filter paper or starch grains) to prevent electro-decantation from occurring during the entry of migrating proteins into the gels. The present paper describes a simple procedure which enables the sample to be ...

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Journal title

volume 17  issue 1

pages  93- 99

publication date 1965-02-01

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